Benchmarking Crop Direct RNA sequencing versus full length IsoSeq

The aim was to explore technological possibilities for direct RNA sequencing using nanopore technology compared to full length cDNA sequencing using Sequel SMRT sequencing. Obtained isoform-aware sequences can be used for improving annotation of a selected crop as well as an isoform reference database for crops of interest within VLPB.
The objective is to explore Nanopore Technology based direct RNA sequencing possibilities and benchmark this technology with the current widely used Pacbio IsoSeq sequencing.
This project took advantage on our experience with multiple Iso-seq projects performed on our in-house Pacbio (Sequel) system proven to enable identification of novel isoforms and high throughput full length cDNA analysis.

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